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Immunomonitoring made simple

1-TruCulture-Tube

 

TruCulture Procedure

TruCulture tubes are pre-loaded with cell culture media and immune stimulant(s) or drug candidates. Blood is drawn directly into the TruCulture tube and incubated in a dry heat block. Supernatants are collected by simply inserting a valve separator to separate cells from the culture supernatant.

 
TruCulture Instructional Video - Vacuum (Preferred)

Blood collection into TruCulture tubes can be conducted either by pulling the plunger before (Vacuum Method) or after (Aspiration Method) connecting the TruCulture tube to the subject. Though both methods are appropriate for the use, the vacuum method is RBM’s phlebotomists preferred method due to ease of use.

 

Collecting TruCulture and additional labs with a priming tube

 

Collecting TruCulture and additional labs when a priming tube isn’t available

TruCulture-Procedure-Collect

 

01. COLLECT

 

Draw 1 mL of blood directly into the TruCulture Tube and break off the plunger.

TruCulture-Procedure-MIX

 

02. MIX

 

Gently invert tube to mix 3 to 5 times.
TruCulture-Procedure-Incubate

 

03. INCUBATE

 

Place tube in 37ºC heat block for up to 24 or 48 hours.

TruCulture-Procedure-separate

 

04. SEPARATE

 

Manually insert valve to separate supernatant from the cells. Collect supernatant and cell layer for downstream analysis.

TruCulture Tube Components

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