Immunomonitoring made simple
Benefits of TruCulture
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Integrated closed sterile instant whole blood collection and culture system.
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Standardized to ensure consistent performance across multiple users and clinical sites.
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Reliable, easy to use, and reproducible – eliminates the need for cell manipulation.
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Retains all blood components, granulocytes, platelets, red blood cells, soluble factors and Fc receptor expressing cells.
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Only inexpensive heat block needed, no lab equipment nor centrifugation steps.
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Has been successfully deployed in hundreds of clinical drug trials.
Disadvantages of Traditional PBMC
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Separate blood collection and specialized cell culture procedures.
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Extensive manipulation, processing, and often freezing/shipping prior to culturing.
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Requires technical expertise with increased variability across users and clinical sites.
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Requires CO2 incubator, biosafety cabinet, centrifuge, media, and cell culture plastics.
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Culture procedures/conditions are difficult to standardize for clinical trial applications.
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Open, less sterile, artificial system.
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Poor reproducibility.
Traditional pharmacodynamic whole blood experiments are generally short in duration (2-6 hrs) as a consequence of poor culture conditions leading to premature termination of the normal physiological immune response. A longer more robust response usually provides higher sensitivity and greater relevance than short incubation times that may only lead to the release of stored pre-synthesized mediators.
