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Meso Scale Discovery (MSD) V-PLEX®

Now available from Rules-Based Medicine

Rules-Based Medicine performs testing on the Meso Scale Discovery (MSD) platform, designed for high-performance biomarker detection.

 

RBM offers the MSD V-PLEX® multiplexed assays, which complement RBM’s other immunoassay platforms. Customers can combine multiplex panels with ultrasensitive single-plex assays for broad biomarker discovery.

 

Key Features

 

  • Multiplexing efficiency: V-PLEX assays use MSD’s multi-spot plates, allowing simultaneous measurement of up to 10 analytes per well.

  • Preconfigured panels: Panels are grouped by biological relevance with validated performance specifications.

  • Reproducibility: V-PLEX is ideal for clinical-grade data generation and high throughput for large-scale studies. Quality parameters include assessing assay sensitivity, precision, specificity, recovery, linearity and repeatability across sample types.

  • In addition to standard specimen types, V-PLEX panels can also be tested using our proprietary TruCulture® tubes.

Available V-PLEX panels for human testing include:

Available V-PLEX panels and performance characteristics*

*Parameters generated by MSD 

Chemokine Panel 1 – Gen B kit

Analyte

LLOD

LLOQ

ULOQ

Dynamic Range

Unit

Eotaxin

0.20

2.14

910

0.20 - 910

pg/mL

Eotaxin-3

1.44

11.1

11,100

1.44 - 11,100

pg/mL

IP-10

0.12

1.29

1,240

0.12 - 1,240

pg/mL

MCP-1

0.16

1.45

247

0.16 - 247

pg/mL

MCP-4

0.05

0.518

234

0.05 - 0.518

pg/mL

MDC

1.00

6.43

2,930

1.00 - 2,930

pg/mL

MIP-1α

0.05

0.357

403

0.05 - 403

pg/mL

MIP-1β

0.09

1.20

390

0.09 - 390

pg/mL

TARC

0.11

2.14

481

0.11 - 481

pg/mL

 

Cytokine Panel 1

Analyte

LLOD

LLOQ

ULOQ

Dynamic Range

Unit

GM-CSF

0.16

0.842

750

0.16 - 750

pg/mL

IL-1α

0.09

2.85

278

0.09 - 278

pg/mL

IL-5

0.14

4.41

562

0.14 - 562

pg/mL

IL-7

0.12

0.851

563

0.12 - 563

pg/mL

IL-12/IL-23p40

0.33

1.32

2,250

0.33 - 2,250

pg/mL

IL-15

0.15

0.774

525

0.15 - 525

pg/mL

IL-16

2.83

19.1

1,870

2.83 - 1,870

pg/mL

IL-17A

0.31

3.19

3,650

0.31 - 3,650

pg/mL

TNF-β

0.08

0.465

458

0.08 - 458

pg/mL

VEGF-A

1.12

7.7

562

1.12 - 562

pg/mL

 

Proinflammatory Panel 1

Analyte

LLOD

LLOQ

ULOQ

Dynamic Range

Unit

IFN-γ

0.37

1.76

938

0.37 - 938

pg/mL

IL-1β

0.05

0.646

375

0.05 - 375

pg/mL

IL-2

0.09

0.89

938

0.09 - 938

pg/mL

IL-4

0.02

0.218

158

0.02 - 158

pg/mL

IL-6

0.06

0.633

488

0.06 - 488

pg/mL

IL-8

0.07

0.591

375

0.07 - 375

pg/mL

IL-10

0.04

0.298

233

0.04 - 233

pg/mL

IL-12p70

0.11

1.22

315

0.11 - 315

pg/mL

IL-13

0.24

4.21

353

0.24 - 353

pg/mL

TNF-α

0.04

0.69

248

0.04 - 248

pg/mL

 

Sample volume

Panel Volume required*
Chemokine 1 (Gen B) 100 μL serum or plasma
Cytokine 1 125 μL serum or plasma
Proinflammatory 1 125 μL serum or plasma

*Ask client services for volume requirements for other acceptable sample types, including CSF, urine, cell culture supernatant, TruCulture supernatant

Acceptable sample types

Serum, plasma, CSF, urine, cell culture supernatant, TruCulture supernatant

For research use only.

 

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