Tumor Necrosis Factor alpha Simoa

Tumor Necrosis Factor alpha (TNF-a, also known as cachexin, or cachectin) is a systemic inflammatory response cytokine and is secreted primarily by macrophages and monocytes during acute inflammation. It is also produced by CD4+ lymphocytes, NK cells, neutrophils mast cells, eosinophils, and neurons. TNF is produced as a type II transmembrane protein. The metalloprotease TNFa converting enzyme (TACE, also known as ADAM17) releases the homotrimeric cytokine from the membrane-bound form through proteolytic cleavage. The membrane-bound and soluble forms of the homotrimer bind to TNFR-1, expressed on most tissues. The membrane-bound form can also bind to TNFR-2, expressed primarily on immune cells. TNF plays a critical role in resistance to bacterial, viral, and parasitic infections and is responsible for a wide range of cellular process, including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF regulates immune cells. As an endogenous pyrogen, TNF induces fever, apoptosis, and can inhibit tumorigenesis. The cytokine has been implicated in a multiple disease states, including autoimmune diseases, insulin resistance, and cancer. TNF dysregulation has been implicated in Alzheimer?s disease and psoriasis.

Swiss-Prot Accession Number: P01375